Today, we learned about a type of DNA sequencing used in the First Generation. The DNA is first denatured by increasing the temperature. The temperature is then decreased for the primer to attach to one strand. DNA polymerase continues adding DNA nucleotides until the fluorescence-stained dideoxyribonucleotide (dNTP) is attached. The process is repeated multiple times in order for the dNTPs to attach at different areas on the DNA strand. These labeled dNTPs are read to sequence the DNA by megabasepairs (Mbp). The price for sequencing DNA was \$10000 in 2001. Technology continued to improve, so the price lowered to \$0.10 in 2011 and to \$0.01 in 2021. This Jupyter Notebook calculates the total cost it would've taken to sequence the sum of the number of basepairs in all of the 23 chromosomes in 2001, 2011, and 2021. classAI-25-07-10